This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.
Mouse Monoclonal Antibody
Purified recombinant fragment of human CD307A (AA: extra 17-202) expressed in E. Coli.
Fc receptor homolog 1 antibody
Fc receptor like protein 1 antibody
Fc receptor-like protein 1 antibody
FcR like protein 1 antibody
FcR-like protein 1 antibody
FCRH 1 antibody
FCRL 1 antibody
hIFGP 1 antibody
IFGP 1 antibody
IFGP family protein 1 antibody
Immune receptor translocation associated protein 5 antibody
Immune receptor translocation-associated protein 5 antibody
immunoglobulin superfamily Fc receptor, gp42 antibody
IRTA 5 antibody
RP11 367J7.7 antibody
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*PBS with 0.05% sodium azide.
Protein G purified.
Fig1: Western blot analysis of CD307A against human CD307A (AA: extra 17-202) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of CD307A against HEK293 (1) and CD307A (AA: extra 17-202)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Flow cytometric analysis of CD307A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-96, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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